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. Author manuscript; available in PMC: 2013 Sep 4.
Published in final edited form as: Eur J Pharmacol. 2011 Mar 31;668(3):428–434. doi: 10.1016/j.ejphar.2011.03.022

Fig. 3.

Fig. 3

Antibody directed against CXCL8 does not block N-ac-PGP-induced calcium mobilization in PMNs. 2.25 × 105 freshly isolated PMNs were loaded with FLUO-3AM. Before measuring, medium (A–C and E) or α-CXCL8 (D and F–I) was added to the cells. After 20 s baseline measurement, the cells were stimulated with medium (A), N-ac-PGP (3 × 10−3 M; C and D) CXCL8 (20 ng/ml; E–I) or α-CXCL8 (10 µg/ml; B). The antibody α-CXCL8 does not stimulate the PMN to release calcium into the cytosol (B). N-ac-PGP (C) and CXCL8 (E) induce calcium mobilization. The N-ac-PGP induced calcium mobilization was not influenced by α-CXCL8 incubation (D), whereas the influx induced by CXCL8 could be attenuated dose dependently by α-CXCL8 incubation (0.1–50 µg/ml; F–I).