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. Author manuscript; available in PMC: 2013 Sep 4.
Published in final edited form as: Eur J Pharmacol. 2011 Mar 31;668(3):428–434. doi: 10.1016/j.ejphar.2011.03.022

Fig. 4.

Fig. 4

CXCL8 is not responsible for N-ac-PGP induced chemotaxis in vitro. 2 × 105 freshly isolated neutrophils were incubated for one hour at 37 °C with various concentrations of α-CXCL8 (0.01–10 µg/ml). The cells were placed in the top well and the chemo-attractant (N-ac-PGP; 3 × 10−3 M or CXCL8; 10 ng/ml) was present in the bottom well of a 96-wells Millipore Filtration Plate System. After one hour incubation at 37 °C, the cells were counted with FACS. CXCL8 (A) and N-ac-PGP (B) induce chemotaxis in PMNs (***, P<0.001 vs. control). The chemotaxis induced by N-ac-PGP is not inhibited by α-CXCL8 (B), whereas incubation of the PMNs with the antibody resulted in a complete inhibition of the CXCL8 induced chemotaxis (A) (^^^, P<0.001 α-CXCL vs. CXCL8).