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. Author manuscript; available in PMC: 2013 Sep 4.
Published in final edited form as: Eur J Pharmacol. 2011 Mar 31;668(3):428–434. doi: 10.1016/j.ejphar.2011.03.022

Fig. 5.

Fig. 5

LPS is not chemotactic for PMNs in vitro. 2 × 105 freshly isolated neutrophils were placed in the top well and the chemo-attractant was present in the bottom well of a 96-wells Millipore Filtration Plate System. After one hour incubation at 37 °C in 5% CO2, the cells from each bottom well were counted. Data were standardized to a chemotactic index. LPS did not induce chemotaxis in PMNs. Concentrations from 100 ng/ml to 100 µg/ml led to a significantly lower chemotactic index, possibly due to cell death by overstimulation (*, P<0.05; **, P<0.01; ***/^^^, P<0.001 LPS/CXCL8 vs. control).