Table 1.

Cell viability of PMNs after N-ac-PGP, PGG and LPS incubation.

	1 h	2 h	4 h	6 h	8 h	19 h	24 h
Control
%PMNs	95.7	95.6	95.5	94.3	93.0	86.2	76.4
% PI+ PMNs	2.7	1.9	2.3	2.8	3.9	21.5	41.6
N-ac-PGP 3 × 10−4 M
%PMNs	96.7	96.6	95.6	95.0	94.7	87.3	78.7
% PI+ PMNs	2.2	2.0	2.7	2.5	3.5	24.9	49.4
N-ac-PGP 10−3 M
%PMNs	97.0	96.6	95.9	94.8	94.9	89.8	84.1
% PI+ PMNs	2.0	1.9	2.4	2.4	3.9	19.5	45.8
N-ac-PGP 3 × 10−3 M
%PMNs	96.9	96.5	95.7	95.1	95.1	91.5	87.4
% PI+ PMNs	2.1	2.0	2.1	2.6	3.3	13.2	34.2
PGG 3 × 10−3 M
%PMNs	97.1	96.5	96.3	94.5	93.7	86.7	76.6
% PI+ PMNs	2.0	2.0	2.1	2.6	3.0	22.9	45.4
LPS 1 µg/ml
%PMNs	86.0	94.2	90.7	88.3	86.9	60.5	47.4
% PI+ PMNs	9.1	4.2	5.5	6.0	9.2	49.8	74.4

7 × 10⁵ buffy coat isolated PMNs resuspended in 300 µl RPMI medium with 1% FCS were loaded with 3 µl PI (5 µg/ml). A gate based on PMNs physical parameters (forward and side scatter) was set to exclude debris. Within this gate, the PI positive population was monitored in the FL2 channel.