Table 3.

ΔSASA values determined from thermodynamic parameters for the binding of various wildtype BH3 peptides to Bcl2 in Sodium phosphate buffer containing 100mM NaCl at 25°C and pH 7

Peptide	Sequence	ΔH60 / kcal.mol−1	ΔCp / kcal.mol−1.K−1	ΔSASApolar / Å2	ΔSASAapolar / Å2	ΔSASAtotal / Å2
Class I: LXXXAD motif
Bmf	QAEVQIARKLQCIADQFHRL	−11.30 ± 0.36	−0.269 ± 0.009	−666 ± 22	−1133 ± 38	−1800 ± 60
Puma	QWAREIGAQLRRMADDLNAQ	−18.41 ± 1.24	−0.352 ± 0.012	−1002 ± 57	−1538 ± 65	−2540 ± 122
Class II: LXXX[G/S]D motif
Bak	STMGQVGRQLAIIGDDINRR	−24.25 ± 0.71	−0.391 ± 0.011	−1250 ± 36	−1768 ± 49	−3017 ± 84
Bim	RPEIWIAQELRRIGDEFNAY	−11.93 ± 0.25	−0.302 ± 0.006	−720 ± 16	−1261 ± 26	−1981 ± 42
Bid	DIIRNIARHLAQVGDSMDRS	−11.65 ± 0.18	−0.211 ± 0.002	−623 ± 9	−932 ± 12	−1555 ± 22
Bax	ASTKKLSESLKRIGDELDSN	−14.20 ± 0.36	−0.203 ± 0.004	−707 ± 18	−942 ± 23	−1649 ± 40
Bik	EGSDALALRLACIGDEMDVS	−13.38 ± 0.21	−0.209 ± 0.001	−684 ± 9	−952 ± 8	−1636 ± 18
Bad	WAAQRYGRELRRMSDEFVDS	−10.18 ± 0.13	−0.185 ± 0.001	−545 ± 6	−816 ± 7	−1361 ± 13
Hrk	SAAQLTAARLKALGDELHQR	−7.94 ± 0.35	−0.193 ± 0.005	−471 ± 18	−809 ± 25	−1281 ± 44

Errors were calculated from at least three independent measurements to one standard deviation.