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Collagen type II formation in MSC 3D cultures collected at 28 days. (A, B) 2.5×105 pellet, (C, D) 5×105 pellet (E, F) alginate, (G, H) FA, and (I, J) agarose. Collagen type II has formed throughout the FA and pellet cultures, but is not evident in alginate culture. Black box represents area for magnified image adjacent to original in all paired images. (K) Serial section from (C) after reaction with nonimmune serum. Scale bar=200 μm.
