FIG. 2.

Reverse transcriptase–polymerase chain reaction (RT-PCR) detection of alkaline phosphatase (ALP) and runt-related transcription factor-2 (RUNX-2) expression. (A) Representative images of three repeated experiments. Lane 1: negative control, MC3T3-E1 cells treated without BRS for 7 days; lane 2: negative control, 14 days; lane 3: MC3T3-E1 cells treated with BRS for 7 days; lane 4: 14 days. (B) Densitometric quantification of RUNX-2 and ALP mRNAs expressed as the ratio of β-actin expression in the same sample (^ap<0.05 vs. control). Color images available online at www.liebertpub.com/tea