FIG. 5.

Analysis of matrix mineralization of MC3T3-E1 cells by Alizarin Red S staining. (A) MC3T3-E1 cells treated with the BRS (S) and without BRS (N) in the presence of LY294002 (0, 0 μM; 1, 5 μM; 2, 10 μM; and 3, 20 μM). Values represent the mean±SD (*p<0.05 vs. S0; ^#p<0.05 vs. N0). (B) MC3T3-E1 cells transfected with AKT2 RNAi plasmids. WT: normal MC3T3-E1 cells; NS: cells transfected with scrambled RNAi; SH1: AKT2-1. Values represent the mean±SD (*p<0.05 vs. NS). Color images available online at www.liebertpub.com/tea