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. 2013 May 30;19(19-20):2253–2266. doi: 10.1089/ten.tea.2012.0649

FIG. 1.

FIG. 1.

Differential expression of NG2 and CD146 in multipotent colonies (OACs) and parental mesenchymal stem cells (MSCs). Representative histograms (A–D) were generated from flow cytometric analysis of 10,000 cells labeled with a cocktail of anti-NG2 APC and anti-CD146 FITC or isotype controls. Surface expression of NG2 (A) and CD146 (B) was analyzed in pooled samples of five single cell-derived cultures of P2 OACs (dark grey) relative to parental MSCs (light gray) and isotypes (black). Corresponding bivariate histograms for OACs (C) and parental MSCs (D). Mean fluorescence intensity (MFI, E) for NG2 (white) and CD146 (black) surface expression is presented as a ratio relative to the MFI of the isotype. Data are reported as a mean±standard error of the mean (n=4). *p<0.05 versus parent.