FIG. 2.

Loss of NG2 and CD146 expression during differentiation. Pooled P2 OAC cultures from five colonies were inoculated at 2×10³ cells/cm² into six-well plates containing 2 mL growth medium/well. Confluent cultures from a single donor were induced to differentiate on day 3 with adipogenic medium (circles) or maintained in growth medium for 25 days as control (triangles). Surface expression of NG2 (A) and CD146 (B) was monitored by flow cytometry and is reported as an MFI ratio as in Figure 1. Lipid accumulation (C) presented as a standardized score of AdipoRed staining as in Supplementary Figure S1. Data are expressed as a mean±standard error of the mean (n=3). *p<0.05 and **p<0.01 versus confluent control on the same day.