Fig. 4.

Regulation of fibrosis-relevant genes in skin of Mep1a^−/− and Mep1b^−/− mice. Relative expression of selected genes in the skin of KO and WT mice. (A–D) A total of 84 genes were quantified in duplicate from two different animals of each genotype using a mouse fibrosis PCR array. (A) Color code indicating the extent of change in KO and WT mice (set as 1.0). (B–D) Details of expression of genes relevant for collagen assembly. (E and F) Relative expression of BMP-1/mTLD mRNA in the skin of Mep1a^−/− and Mep1b^−/− mice (E) and in corresponding primary fibroblasts (F) analyzed by quantitative real-time PCR. n = 3. *P < 0.05; **P < 0.01; ***P < 0.001. Error bars show SD.