Fig. 3.

XNC10shRNA Tg Xenopus tadpoles have reduced expression of iVα6. Expression of (A) XNC10 or (B) iVα6 in Tg developmental stage 53/54 tadpoles (white box) or age-matched dejellied controls (black box). Results are normalized to an endodgenous control and presented as fold change in expression compared with expression of XNC10 or iVα6 in the skin of Tg tadpoles. All results are presented as mean ± SE (n = 4). *P < 0.05 and **P < 0.005 denote significant differences (Student t test). (C) F0 Tg tadpoles (stage 53, n = 12) or control larvae (stage 53, n = 15) were i.p. infected with 1 × 10⁴ pfu FV3, and survival was monitored daily. Active FV3 infection was verified postmortem by RT-PCR using viral polymerase II on kidney-derived genomic DNA. OB, outbred animals; RQ, relative quantification.