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. 2013 Aug 12;110(35):14366–14371. doi: 10.1073/pnas.1304230110

Fig. 1.

Fig. 1.

WNK1+/FHHt mice display signs of hypervolemia. (A) Quantification of the expression level of Renin transcripts in the kidney cortex of mice WNK1+/FHHt (n = 7) and control littermates (WNK1+/i1lox; n = 6) by real-time RT-qPCR showed that expression of Renin is significantly decreased in WNK1+/FHHt males (**P < 0.01). Results (mean ± SEM) are expressed in arbitrary units relative to the expression of ubc, and the expression level in WNK1+/i1lox mice was arbitrarily set to one. (B) Profiles over 24 h in SBP under a 12:12-h day:night (7:00 AM to 7:00 PM and 7:00 PM to 7:00 AM) schedule in WNK1+/i1lox (n = 7) and WNK1+/FHHt (n = 6) mice kept under a control diet (NNa; 0.3% of Na+) instrumented with a telemetric system under basal condition. Data are mean ± SEM, and each point corresponds to a 1-h average. (C) SBP is increased in WNK1+/FHHt mice (n = 6) compared with WNK1+/i1lox littermates (n = 7). Data correspond to the 12-h night period mean ± SEM. *P < 0.05 (repeated measures two-way ANOVA). (D–F) Twelve-hour average SBP (night period) was slightly and transiently increased by administration of a high salt diet (HNa; 3% NaCl) in WNK1+/FHHt mice, whereas the SBP of WNK1+/i1lox mice was not affected (E). Data are mean ± SEM. *P < 0.05 compared with WNK1+/FHHt kept under NNa (Dunnett multiple comparisons test after a one-way repeated measures ANOVA). (F) Twelve-hour average SBP (night period) was normalized in WNK1+/FHHt mice by a hydrochlorothiazide treatment during 3 d, whereas the SBP of WNK1+/i1lox mice was not modified. **P < 0.01 compared with WNK1+/FHHt mice kept under a HNa (paired Student t test). For all panels, data correspond to the 12-h night period mean ± SEM.