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. 2013 Aug 12;110(35):14366–14371. doi: 10.1073/pnas.1304230110

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Fig. 4. — The abundance of the phosphorylated form of SPAK is not affected in homogenates of kidney cortex from WNK1^+/FHHt mice. (Left) Representative immunoblots performed on renal cortex homogenates from control and WNK1^+/FHHt males (n = 7 per group) incubated with antibodies raised against SPAK and the phosphorylated SPAK/OSR1 S motif. (Right) Densitometric analysis showed that the abundance of SPAK and its phosphorylated form on Ser343 is not modified in homogenates of cortex isolated from WNK1^+/FHHt kidneys compared with control kidneys. Data are mean ± SEM.