Fig. 3.

Effect of SAMHD1 silencing on the growth of lung fibroblasts. (A) Cell growth in cultures transfected for 24–72 h with anti-SAMHD1 (open circle) or control (filled circle) siRNAs. Data from three experiments. Bars are SEs. (B) Percent of G1 (black), S (gray), and G2/M (white) cells in transfected cultures. The frequency of S-phase cells is indicated above each control (C) and silenced (Si) sample. (C) Frequency of BrdU-positive cells in cultures transfected with control (filled circle) or anti-SAMHD1 (open circle) siRNAs for 18–48 h and incubated with BrdU during the last 30 min. Data are from two identical experiments. At least 500 cells were scored for each time point. The frequencies were compared by 2 × 2 χ² analysis. *P < 0.05; **P < 0.001. Bars are SEs.