Fig. 4.
Induction of PI3KH1047R promotes mammosphere formation. (A) Mammospheres were imaged using the 10× objective of an Olympus CK40 microscope. (Scale bar, 100 µm.) (B) Total mammosphere volume from each well was quantified using Gelcount software on day 13. (C) DOX-treated HER2+PIK3CA mammospheres were treated with 20 µg/mL trastuzumab, 1 µM BKM120, or the combination. Total mammosphere volume from each well was quantified using Gelcount software on day 12. Data represent the average ± SD of three replicate wells. **P < 0.01, Student t test.