Fig. 9.

MyD88 deficiency in DCs rescues the phenotype of lyn^f/f Cd11c-cre⁺ mice. (A) lyn^f/f myd88^f/f Cd11c-cre⁺ mice were euthanized at 8 mo of age and their kidneys were analyzed by histology for signs of nephritis (H&E staining). Representative picture is shown. Scale bars, 100 μm. (B) Autoreactive antibodies in the sera from control, lyn^f/f Cd11c-cre⁺, and lyn^f/f myd88^f/f Cd11c-cre⁺ mice of the indicated ages determined by ELISA. Data represent mean ± SEM from 8–12 mice per group. *P ≤ 0.05 compared with control and to lyn^f/f myd88^f/f Cd11c-cre⁺ (Kruskal–Wallis test). (C and D) Splenic and cervical lymph node weights from 8-mo-old control, lyn^f/f Cd11c-cre⁺, and lyn^f/f myd88^f/f Cd11c-cre⁺ mice are reported. The absolute numbers of splenic myeloid cells (E), plasmacytoid DCs (F), conventional DCs (G), effector T cells (J), and plasma cells (K) as determined by flow cytometry are shown. Data represent mean of independent flow cytometry experiments. Each dot represents an individual mouse. Open circles, control; red circles, lyn^f/f Cd11c-cre⁺; green circles, lyn^f/f myd88^f/f Cd11c-cre⁺. (H) FACS histogram showing the expression level of CD86 by CD11c^hi splenic DCs from 8-mo-old mice. (I) MFI (relative to control) of CD86 expressed by splenic CD11c^hi DCs from 8-mo-old mice. Bars represent mean ± SEM of independent experiments from 8–12 mice per group. (C–K) Data from control and lyn^f/f Cd11c-cre⁺ groups are the same as represented in Figs. 3–7, to perform comparisons to the lyn^f/f myd88^f/f Cd11c-cre⁺ mice. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001 (Kruskal–Wallis test).