Fig. 1.

R-spo3 attenuates mesenteric I/R-induced local intestinal and remote lung injury. Representative images of hematoxylin and eosin stained intestinal sections of mice from groups (A) sham, (B) sham + R-spo3, (C) I/R, and (D) I/R + R-spo3 (n = 5 per group) are shown. Arrowheads (C) emphasize villi damage characterized by exudation of lamina propria. (E) Average score of intestinal injury for each group was quantified using a scoring system (SI Experimental Procedures). Representative images of hematoxylin and eosin stained lung sections of mice from groups (F) sham, (G) sham + R-spo3, (H) I/R, and (I) I/R + R-spo3 (n = 5 per group) are shown. Arrowheads (H) emphasize lung damage characterized by alveolar/interstitial infiltration. (J) Average score of lung injury for each group was determined based on the criteria described in SI Experimental Procedures. The extent of I/R is 30/180 min. All photomicrographs are 200× magnification. Error bars represent SEM (**P < 0.01, ***P < 0.005). Results are representative of three independent experiments.