Fig. 6.

T_M cells have enhanced glycolysis, more ATP, and proliferate faster than T_N cells after activation. CD8 T_N (CD44^lo CD62L^hi) and T_M (CD44^hi CD62L^hi) cells were isolated from naïve and LmOVA-infected mice. OCR and ECAR in T_N and T_M cells after stimulation with aCD3/28 coated beads (A), and after subsequent oligo (1 μM), FCCP (1.5 μM), and rotenone (100 nM) plus antimycin A (1 µM) injections (B) (data in A and B are from the same experiment). (C) T_N and T_M cells were PMA/iono stimulated, exposed to oligo + FCCP, and R+A, and OCR and ECAR measured. T_N is the same as Fig. S4, T_M is the same as Fig. 8F and Fig. S7. (D) Compiled and baselined data as shown in C, from two experiments; peak is first measurement after PMA/iono, plateau is at 120 min. *P < 0.0005 (Left), and < 0.0001 (Right). Forward scatter and ATP of resting T_N and T_M cells (E), and ATP in primary and secondary T_E cells 3 and 24 h after aCD3/28 (F). (G) T_N and T_M cells stimulated with aCD3/28 with or without oligo (day 0) and proliferation is shown. T_N control is the same as in Figs. S6B and S12B. T_M control is the same as Fig. 7A and 8E; mean ± SEM, representative of two (A, B, and E), 3 (C), or one (F and G) experiment(s).