Fig. 3.
Lin28a overexpression suppresses let-7 expression and derepresses let-7 target genes in chondrocytes. (A) miRNA expression profiles in primary rib chondrocytes of Cre-negative Ctrl and Col2-Cre:Lin28ac (Lin28-Tg) mice. Expression levels of 165 miRNAs detected in chondrocytes were normalized by that of the U6 level. Expression levels of miRNAs in Lin28-Tg chondrocytes are shown as fold differences relative to those of controls. The let-7 miRNAs were relatively specifically down-regulated in Lin28-Tg, except for let-7c (arrow). (B) Target let-7 genes were up-regulated in Lin28-Tg rib chondrocytes. Some of microarray results (Table S1) were verified by qRT-PCR (n = 3; *P < 0.05 vs. Ctrl). (C) Up-regulation of CDC34 and E2F5 proteins in primary rib chondrocytes of Lin28-Tg mice was verified.