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. Author manuscript; available in PMC: 2013 Sep 4.
Published in final edited form as: Traffic. 2008 Apr 11;9(7):1130–1145. doi: 10.1111/j.1600-0854.2008.00747.x

Figure 3. MARCH7 associates with the DUBs USP9X and USP7.

Figure 3

A, B) MARCH7 co-immunoprecipitates with USP7 and USP9X. HEK-293T cells were co-transfected with MARCH7-ZZ/MARCH7 WI-ZZ and (A) USP9X/USP9X CS or (B) USP7/USP7 CS, lysed in 1% Triton X-100 and MARCH7 immunoprecipitated on IgG sepharose beads. 1×106 cells equivalents were loaded per lane, separated by SDS-PAGE and immunoblotted for USP9X, USP7 and MARCH7 (c-myc antibody). 1×105 cells/lane of lysate were loaded and immunoblotted for calreticulin (Cal) as a loading control.