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. Author manuscript; available in PMC: 2013 Sep 4.
Published in final edited form as: Cell Calcium. 2013 May 20;54(2):86–94. doi: 10.1016/j.ceca.2013.04.006

Fig. 4.

Fig. 4

Effect of mPhaZ7 expression on mitochondrial calcium uptake. Expression of mPhaZ7 inhibits mitochondrial calcium uptake in the intact HepG2 (A) and HeLa (B) cells induced by the addition of 100 µM ATP. The expression of inactive point mutant of mPhaZ7 (Ser136Ala) labeled as mPhaZ7-PM, did not inhibit calcium uptake (C). Application of calcium to mitochondria of digitonin-permeabilized cells induces uptake of this cation in control but not in mPhaZ7-expressing mitochondria in HepG2 (D) and HeLa (E) cells. Ferutinin was used at a concentration 20 µM. In panel (E) calcium concentrations are in nM.