Table 1.
MxiHΔ5 | Freshly Prepared | 2 wks, 4 °C | 2 wks, 40 °C |
---|---|---|---|
Prior to Elution | 0.001 | 0.001 | 0.000 |
10 mM Histidine | 0.003 | 0.002 | 0.014 |
0.5 M NaCl | 0.001 | - | - |
0.75 M NaCl | 0.002 | - | - |
1.0 M NaCl | 0.002 | - | - |
2.0 NaCl | 0.005 | - | - |
3.0 M NaCl | 0.001 | 0.000 | - |
1.0 M Gdn HCl | 0.003 | 0.003 | 0.001 |
1.0 M NaCit | 0.143 | 0.091 | 0.039 |
1.0 M NaPhos | 0.201 | 0.160 | 0.078 |
70 mM SDS | 0.088 | - | - |
PrgIΔ5 | |||
Prior to Elution | 0.001 | 0.002 | 0.002 |
10 mM Histidine | 0.003 | 0.003 | 0.000 |
0.5 M NaCl | 0.001 | - | - |
0.75 M NaCl | 0.002 | - | - |
1.0 M NaCl | 0.004 | - | - |
2.0 NaCl | 0.003 | - | - |
3.0 M NaCl | 0.000 | 0.006 | - |
1.0 M Gdn HCl | 0.005 | 0.006 | 0.004 |
1.0 M NaCit | 0.137 | 0.081 | 0.036 |
1.0 M NaPhos | 0.197 | 0.151 | 0.092 |
70 mM SDS | 0.056 | - | - |
BsaLΔ5 | |||
Prior to Elution | 0.001 | 0.002 | 0.002 |
10 mM Histidine | 0.004 | 0.003 | 0.003 |
0.5 M NaCl | 0.000 | - | - |
0.75 M NaCl | 0.001 | - | - |
1.0 M NaCl | 0.006 | - | - |
2.0 NaCl | 0.003 | - | - |
3.0 M NaCl | 0.000 | 0.000 | - |
1.0 M Gdn HCl | 0.002 | 0.005 | 0.002 |
1.0 M NaCit | 0.171 | 0.105 | 0.036 |
1.0 M NaPhos | 0.196 | 0.154 | 0.082 |
70 mM SDS | 0.100 | - | - |