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. Author manuscript; available in PMC: 2013 Sep 4.
Published in final edited form as: J Pharm Sci. 2010 Nov;99(11):4488–4496. doi: 10.1002/jps.22180

Table 1.

Protein (mg/mL) present in sample supernatant as assayed by UV absorbance spectroscopy following ‘elution’ treatment. All samples originally contained 0.2 mg/mL protein in the presence of 0.5 mg/mL Aluminum. Where a dash is present no sample was analyzed. Error was estimated at approximately 0.05 mg/mL.

MxiHΔ5 Freshly Prepared 2 wks, 4 °C 2 wks, 40 °C
Prior to Elution 0.001 0.001 0.000
10 mM Histidine 0.003 0.002 0.014
0.5 M NaCl 0.001 - -
0.75 M NaCl 0.002 - -
1.0 M NaCl 0.002 - -
2.0 NaCl 0.005 - -
3.0 M NaCl 0.001 0.000 -
1.0 M Gdn HCl 0.003 0.003 0.001
1.0 M NaCit 0.143 0.091 0.039
1.0 M NaPhos 0.201 0.160 0.078
70 mM SDS 0.088 - -
PrgIΔ5
Prior to Elution 0.001 0.002 0.002
10 mM Histidine 0.003 0.003 0.000
0.5 M NaCl 0.001 - -
0.75 M NaCl 0.002 - -
1.0 M NaCl 0.004 - -
2.0 NaCl 0.003 - -
3.0 M NaCl 0.000 0.006 -
1.0 M Gdn HCl 0.005 0.006 0.004
1.0 M NaCit 0.137 0.081 0.036
1.0 M NaPhos 0.197 0.151 0.092
70 mM SDS 0.056 - -
BsaLΔ5
Prior to Elution 0.001 0.002 0.002
10 mM Histidine 0.004 0.003 0.003
0.5 M NaCl 0.000 - -
0.75 M NaCl 0.001 - -
1.0 M NaCl 0.006 - -
2.0 NaCl 0.003 - -
3.0 M NaCl 0.000 0.000 -
1.0 M Gdn HCl 0.002 0.005 0.002
1.0 M NaCit 0.171 0.105 0.036
1.0 M NaPhos 0.196 0.154 0.082
70 mM SDS 0.100 - -