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Confirmation of known CanScript-binding candidates. TEAD1, YAP1, SP1, KLF6 and TEAD2 were examined for their binding to DNA (wild-type or transcriptionally inactive C5Mc6 mutant CanScript ×3) by immunoblot analysis after DNA affnity-capture. For each blot, one-third of the final captured sample was resolved by SDS-PAGE. 2% of the nuclear extract was used as the input reference. WT, wild-type.
