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Dependence of MSLN expression on expression of three interactome genes. siRNA treatments of (A) NAB1, (B) RFX1 and (C) ZNF24 were examined for knockdown efficacy and for effects on MSLN expression in HeLa cells. Each gene was tested with three nonoverlapping siRNA sequences. Irrelevant target FANCD2 (Irr) and TEAD1 siRNA were used as negative and positive control, respectively. Alpha-tubulin was used as a comparison for sample loading.
