Table 1.

Proteins Binding Canscript Sequence as Identified by SD-MS

accession	gene IDa	# of peptides identified	times identifiedb	ratio (heavy/light, WT/MT)c
NP_003205.2	TEAD3d	10	3/3	≥100
NP_068780.2	TEAD1d,e	12	3/3	≥93
NP_008896.2	ZNF24e	7	3/3	≥41
NP_003204.2	TEAD4d	9	2/3	≥100
NP_004354.2	CEACAM5e	2	2/3	≥100
NP_002909.4	RFX1e	2	2/3	≥54
NP_001129493.1	NFATC2d,e	3	2/3	≥49
NP_005957.2	NAB1d,e	2	1/3	≥40
NP_001091947.1	GRSF1e	2	2/3	≥15

^aFull names of identified proteins are provided in Supporting Information Table S1.

^bSD-MS was replicated three times using independent extracts.

^cCandidates having a WT/MT ratio of at least 10 are listed. WT, Canscript wild-type; MT, Canscript C5M6C mutant.

^dAlso identified this protein or a member of this protein family by TFM.

^eIncluded among the proteins examined by immunoblot after DNA affnity-capture.