Table 2.
Proteins Binding Canscript Sequence as Identified by TFM
| gene ID | ratio (WT/MT)a | identified protein full name |
|---|---|---|
| NFATc1b,c | 33.5 | nuclear factor of activated T cells, cytoplasmic 1 |
| DHX36 | 29.3 | deah box polypeptide 36 |
| RBM12 | 29.3 | RNA-binding motif protein 12 |
| SUMO4 | 26 | small ubiquitin-like modifier 4 |
| CAMK2D | 22.3 | calcium/calmodulin-dependent protein kinase II-delta |
| IKZF1c | 18.3 | IKAROS family zinc finger 1 |
| TEAD2b | 15.9 | transcriptional enhancer factor 4 |
| HBP1c | 14.8 | HMG box-containing protein 1 |
| PRDM14c | 13 | PR domain-containing protein 14 |
| NAB1b,c | 12 | NFFI-A-binding protein 1 |
| BZW1 | 11.6 | basic leucine zipper and W2 domains 1 |
| ING2 | 11.3 | inhibitor of growth 2 |
| ACTL6B | 10.6 | actin-like 6B |
| RBM9 | 10.6 | RNA-binding motif protein 9 |
| YY1 | 10.5 | transcription factor YY1 |
| CRAT | 10.4 | carnitine acetyltransferase |
| Tcf15 | 10.2 | transcription factor 15 |
| CD80 | 10.1 | B-lymphocyte activation antigen B7-1 |
a
Candidates having a WT/MT ratio of at least 10 are listed. WT, Canscript wild-type; MT, Canscript C5M6C mutant.
b
Also identified this protein or a member of this protein family by SD-MS.
c
Included among the proteins examined by immunoblot after DNA affnity-capture.