Figure 6. Clodronate depletes Kupffer cells and ameliorates increased T_h1 associated cytokine expression in steatotic liver tissue.

A. Dosing regimen for macrophage depletion studies. Mice with established hepatosteatosis after 10 weeks of CDD were injected every 4-5 days with clodronate for the following 3 weeks, mice after 20 weeks of CDD received clodronate every 4-5 days for the following 5 weeks while continuously fed CDD. B. Liver sections stained with F4/80 antibody from wt mice administered vehicle or clodronate as described in A. Representative photomicrographs at 400x magnification presented. C. Hepatic T_h1 and T_h2 cytokine expression as assessed by real-time PCR in vehicle or clodronate treated animals. Values are mean ± SEM, *p<0.05. n=4-6 animals per group.