Table 2.
Comparisons Between the Original and the Taking-Difference Linear Regression Methods
| |
The original linear regression method with background subtraction |
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|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| |
Mean of cycles 1–3 |
Mean of cycles 3–7 |
Minimum |
The taking-difference linear regression method |
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| DNA molecular numbera | Estimateb | RE | CV | MSE | Estimate | RE | CV | MSE | Estimate | RE | CV | MSE | Estimate | RE | CV | MSE |
| 3.14E+07 | 4.02E+07 | 0.280 | 41.96% | 3.66E+14 | 2.69E+07 | −0.144 | 20.31% | 5.52E+13 | 3.83E+07 | 0.219 | 41.96% | 4.27E+14 | 3.20E+07 | 0.019 | 32.29% | 1.27E+14 |
| 3.14E+06 | 3.21E+06 | 0.022 | 33.22% | 1.17E+12 | 3.17E+06 | 0.009 | 32.04% | 1.10E+12 | 3.49E+06 | 0.111 | 39.91% | 3.06E+12 | 2.85E+06 | −0.093 | 28.90% | 9.21E+11 |
| 3.14E+05 | 2.73E+05 | −0.130 | 35.07% | 1.11E+10 | 2.57E+05 | −0.181 | 34.54% | 1.15E+10 | 4.14E+05 | 0.318 | 66.90% | 1.03E+11 | 2.63E+05 | −0.161 | 26.49% | 8.78E+09 |
| 3.14E+04 | 2.36E+04 | −0.248 | 31.38% | 1.17E+08 | 2.12E+04 | −0.326 | 32.65% | 1.55E+08 | 5.12E+04 | 0.630 | 48.44% | 1.23E+09 | 2.76E+04 | −0.121 | 31.17% | 1.03E+08 |
| 3.14E+03 | 3.44E+03 | 0.097 | 54.00% | 3.56E+06 | 2.96E+03 | −0.059 | 46.90% | 2.00E+06 | 8.62E+03 | 1.746 | 103.70% | 1.20E+08 | 3.51E+03 | 0.118 | 51.24% | 3.61E+06 |
| 3.14E+02 | 9.31E+02 | 1.965 | 126.84% | 1.76E+06 | 6.23E+02 | 0.984 | 87.30% | 3.91E+05 | 5.38E+03 | 16.141 | 410.44% | 5.43E+08 | 3.02E+02 | −0.038 | 36.91% | 1.44E+04 |
| 3.14E+01 | 2.97E+01 | −0.055 | 94.44% | 7.83E+02 | 2.51E+01 | −0.199 | 81.92% | 4.64E+02 | 8.44E+01 | 1.688 | 153.91% | 2.11E+04 | 3.96E+01 | 0.262 | 46.81% | 4.42E+02 |
| Mean | 0.276 | 59.56% | 0.012 | 47.95% | 2.979 | 123.61% | −0.002 | 36.26% | ||||||||
a
Input DNA molecular numbers.
b
Estimated DNA molecular numbers.
CV, coefficient of variation; MSE, mean square error; RE, relative error.