Table 3.
|
|
The original linear regression method |
|
||
---|---|---|---|---|---|
Mean of cycles 1–3 | Mean of cycles 3–7 | Minimum | The taking-difference linear regression method | ||
Amplification efficiency | Overall values | Middle | Largest | Smallest | Middle |
Values >100% | Never | Sometimes | Never | Never | |
Change from 100% to 60% amplification mix | Not monotonically decreasing | Not monotonically decreasing | Not monotonically decreasing | Monotonically decreasing | |
SD | 0.037 | 0.035 | 0.061 | 0.023 | |
Initial DNA amount | RE | Large (up to ∼30%) | Small | Large (up to 300%) | Small |
CV | Up to 60% | Up to ∼50% | Up to ∼120% | Up to ∼35% |
SD, standard deviation.