Table 1.
Primers used in this study
| Primer name | Primer sequence | gene | (a) |
|---|---|---|---|
|
Northern probes | |||
| Zfor |
AAAGTWATCGGTGTCGGCGGWGGC |
ftsZ |
+43 |
| Zrev |
CAGAAATACCTTGAACCCCTTGGCG |
ftsZ |
+595 |
| Ain |
GAACAGCAATGAAATATATGTTG |
ftsA |
+3 |
| N2R |
ACCGTCTACAATGAACTGTC |
ftsA |
+411 |
|
Primer Extension | |||
| prex |
GCCCAAACCGCACTCGCAC |
ftsW |
+95 |
| Wrev |
AATCCATTCTCTGTACCAATG |
murG |
+125 |
| Rip2 |
GTTGCTTAGYAGCCAGTTTC |
murG |
+1030 |
| Qrev |
TCTTTARCTTTGGTACACGATC |
ftsQ |
+52 |
| Arev |
TCATTAACCATTTCACCAATGATG |
ftsA |
+80 |
| N2R |
ACCGTCTACAATGAACTGTC |
ftsA |
+411 |
| ZB |
CACCGTGTTCAATCATACGG |
ftsZ |
+103 |
| ZD |
ACAACCAAACAACGTCGGCG |
spoIIGA |
+74 |
| ZDbis |
CCTAACACAAGCCTCCATC |
spoIIGA |
+158 |
| BigD |
CCCAAATGCTGTATACACAATAAGTAACGAG |
spoIIGA |
+273 |
|
RT-PCR | |||
| Zfin |
CTTTTATCGTCTACGACGGTTAC |
ftsZ |
+1158 |
| Zin |
CATGTTAGAGTTTGATACTACTC |
ftsZ |
−1 |
| Ain |
GAACAGCAATGAAATATATGTTG |
ftsA |
+3 |
| Afin |
CCCATAAATAACGGAATGCACG |
ftsA |
+1297 |
| Qin |
CGTACATGAARAAYAGTAARG |
ftsQ |
−5 |
| Mbin |
GAGATTGTCTATGGAACAATTAG |
murB |
−10 |
| MGin |
ACAGCTGAAACNCTTATTCGTG |
murG |
+964 |
| Fw |
CATCAGCACCGTATCGRATG |
ftsW |
+601 |
|
Mini-ftsZ |
|
|
(b) |
| Hind5 |
GACAAGCTTATATTGGTGTTCGTGAG |
ftsA |
+1056 |
| Eco5 |
GGCGAATTCGCTAATTGATCTTGAG |
ftsZ |
+39 |
| Eco3 |
CACGAATTCAAAACAACGTGAAGTTAAG |
ftsZ |
+1035 |
| Bam3 |
GGCGGATCCAAAAAGGAGCATGAAAGCTC |
spacer |
+28 |
| Amy5 | GCCGCGATTTCCAATGAGG | pJPR1 | +245 |
(a) Position of the primer 5’ nucleotide on the corresponding gene numbering beginning from the first codon of the gene (+1).
(b) Position on the gene of the first complementary primer base after the added restriction site evidenced bold.