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. 2013 Apr 12;13:60. doi: 10.1186/1471-2229-13-60

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Copyright © 2013 Jia et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Amylopectin branch length analysis. Solubilized starch was de-branched in the presence of isoamylase and the resulting glucan chains were separated by capillary electrophoresis. (A) Histograms of the distribution of glucan chains were similar but W64Ao2 was shifted toward a higher degree of polymerization. (B) Difference plot was calculated by subtracting the W64A + values from the W64Ao2 values showed a substantial increase in chains with a degree of polymerization between 15 and 25 glucose subunits. The histograms represent the average of three replicates for each genotype.