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. Author manuscript; available in PMC: 2014 Jan 8.
Published in final edited form as: Curr Biol. 2013 Jun 13;23(13):1163–1172. doi: 10.1016/j.cub.2013.05.008

Figure 5.

Figure 5

441-GAL4 targets redundant neurons necessary for the execution of aversion. (A, A′) 441-GAL4 expression is visualized with a membrane-tagged GFP in the brain (A) and the VNC (A′). Arrowhead: the ellipsoid body. (B, B′) tsh-GAL80 suppresses VNC expression of 441-GAL4. (C) Aversion in 441 > shits1 flies is partially restored with tsh-GAL80 or EB-GAL80. The first bar represents the same data as the last bar in Figure 3B. Bars are mean PIs, and error bars represent s.e.m. (n ≥ 4); t-test with Holm-Bonferroni post hoc correction. (D, D′) R13C06-GAL80 suppresses EB expression of 441-GAL4, as indicated by the open arrowhead. (E–F′) Average 441-GAL4 expression without (E–E′) or with (F–F′) tsh-GAL80 is visualized with a dendritic marker in the ventral (E–F) and dorsal (E′-F′) halves of the VNC after image registration against a standard VNC, shown with neuropil counterstaining of VNC (E1-F1′) or without (E2-F2′). Dashed boxes: abdominal ganglion; arrows: ventromedial cell bodies (E, suppression indicated by dashed arrows in F) and the corresponding dorsal-bilateral projections (E′ suppression indicated by dashed arrows in F′); arrowhead: projection along the midline (E′, suppression indicated by open arrowhead in F′). Scale bars: 50 μm.