Fig. 4. AgNPs promote ROS-induced apoptosis. (A, B) DC2.4 cells were pre-treated with 10 mM NAC for 30 min. The cells were then cultured with AgNPs for 1 h. Subsequently, the cells were stained with annexin V (x-axis) and PI (y-axis). Cell death was analyzed by flow cytometry using a FACSCantoTMII, and the percentages of apoptotic cells are shown in each quadrant (A). (B) The total annexin V-positive cells were quantified.
