Fig. 3. Effects of orally administered GS on Tgase-2 expression in the TPA ear edema model. Three experimental groups included three ICR mice/group. Group 1 received acetone group 2 received TPA group 3 received TPA and GS (10 mg/kg body weight). Ede-ma was induced in both ears of each mouse by topical application of 2.5 μg TPA dissolved in 25 μl of acetone to the inner ear sur-faces. (A) Effect of GS on TPA-induced changes in Tgase-2 protein in mouse ear skin. (B) 25 μl of TPA (100 μg/ml in acetone) was applied to each ear of BALB/c mice and ear biopsy samples were taken at 6 h and subjected to immunostaining with anti-mouse Tgase-2 antibody (1:50 dilution ratio).