Fig. 6. Western blot analysis of PI3K and p-Akt. (A) Activation of signal transduction pathways by H2O2. Hepa-1c1c7 cells were treated with H2O2 over a time course and analyzed by western blotting. (B) Hepa-1c1c7 cells were pretreated with rutaecarpine for 48 h, treated with H2O2 for 3 h, and analyzed by western blotting.
