Fig. 5. NDGA recovered skin barrier and atopic dermatitis in hairless mice. To induce atopic dermatitis, mice were treated with oxazolone 0.05% for 9 days. Then they were treated with vehicle, dexamethasone (0.05%) and NDGA (0.01%, 0.1% and 1%) for additional 25 days. (A) Skin barrier recovery was checked by dorsal TEWL change. The dexamethasone-treated group recovered over 50% of the skin barrier at day 10. NDGA treated mice experienced the best results at day 10. NDGA treatment at 0.01%, 0.1% and 1% showed recovered skin permeability at 18%, 26% and 7%, respectively. At day 22, the NDGA and vehicle-treated groups showed similarly recovery. (B) To measure serum IgE level, blood samples were taken using retro orbital sinus blood collection. After the samples were centrifuged at 10,000 rpm for 10 minutes, supernatants were used in the following experiment. While the vehicle-treated group showed a 15% decrease in serum IgE level, dexamethasone and NDGA (0.01%, 0.1% and 1%)-treated groups showed decreases of 25%, 22%, 5% and 24%, respectively. (C) Dexamethasone causes weight loss and skin thinning when it is used chronically. During our experiment, the dexamethasone-treated group showed a significant decreased in weight. The data are shown as mean or mean ± SEM. *p<0.05, **p<0.01, compared with the control at indicated time.