Fig. 1. Nemadipine-A sensitizes TRAIL-induced cell death in lung cancer cells. (A, B) H1299 and A549 cells were treated with TRAIL (20 ng/ml) with increasing concentration of nemadipine-A (1 to 30 μM) for 8 hours. Cell viability and caspase activation were then determined using the CCK-8 assay. (C) H1299 cells were either untreated (Con.) or treated with TRAIL (20 ng/ml), nemadipine-A (Nema. (20 μM), or a combination of nemadipine-A and TRAIL for 8 hours. The cells were then harvested, and analyzed by western blotting with the indicated antibodies. Actin was used as an internal loading control. The protein levels were measured by densitometry analysis. The data were obtained from at least three different experiments, and are presented ± standard error of the mean (SEM), (n=3, *<0.05).