Fig. 2. (A) Immunofluorescence analysis of core-positive cells in the Huh7.5 cells transfected with either wild type J6/JFH1 or mutant J6/JFH1-79A82A RNAs at 3 days post-transfection. The RNAs-transfected cells were staining with a mouse anti-core antibody and an anti-mouse Alexa 488 antibody. Nucleus in each cell was stained with DAPI. (B) Western blot analysis of JAK1 and core proteins in the Huh7.5 cells mock-transfected or transfected with either wild type J6/JFH1 or mutant J6/JFH1-79A82A RNAs at 3 days ago. (C) Quantitation of the relative viral RNA levels in the cells transfected with either wild type J6/JFH1 or mutant J6/JFH1-79A82A RNAs at 3 days ago by real-time RT-PCR analysis. Average values from triplicate experiments and standard deviations were noted. (D) A renilla luciferase assay using Huh7.5 cells transfected with either a renilla luciferase-linked wild type J6/JFH1 or mutant J6/JFH1-79A82A RNAs at 8 hr and 48 hr post-transfection. Average values from triplicate experiments and standard deviations were noted.