Fig. 5. Examination of multimeric complex formation of the core protein by a sucrose gradient centrifugation followed by Western blot analysis. Cell lysates were prepared from either wild type or mutant J6/JFH1-79A82A RNAs-transfected cells at 3 days post-transfection. The isolated cell lysates were centrifuged together with a sucrose gradient to separate total proteins according to its density. Then, Western blot analysis using an anti-core antibody was performed.