Fig. 3. Confirmation by real-time RT-PCR of mRNA expression patterns of three selected genes. Real-time RT-PCR shows up-regulation of metallothionein 1a, TGF-1 and PPT-2 in cultured DRG cells treated with gabapentin. Data are shown as means +SD (bars) of samples conducted in triplicate determinations. To normalize the efficiency of real-time RT-PCR reaction, β-actin gene was used as an internal standard.
