Fig. 2. The effects of MG132 on cell viability and PAI-1 promoter activity in rat primary astrocytes. (A) Rat primary astrocytes were incubated in serum-free DMEM/F12 with increasing concentration of MG132 (0.1-1 μM) for 24 h. The viability of astrocytes was determined by the MTT reduction assay. (B) Rat primary astrocytes were in-cubated with MG132 (0.1-1 μM) for 24 h after transfection with pGL-hPAI-798 and pCMV-β-galactosidase plasmid. PAI-1 promoter activity was measured as described. All graphs represent the mean ± S.E.M. of four independent experiments (*p<0.05, **p<0.01 versus Control).
