Fig. 5. The effects of MG132 on immunological stimulation in rat primary astrocytes. (A) Rat primary astrocytes were incubated in serum-free DMEM/F12 with increasing concentration of MG132 (0.1-1 μM) and 100 ng/ml LPS for 24 h. Intracellular ROS was measured by H2DCF-DA fluorescence (490/530 nm) as described in Materials and Methods. (B) Rat primary astrocytes were incubated in serum-free DMEM/F12 with 1 μM MG132 and 100 ng/ml LPS for 24 h. Expression of total cellular tPA, PAI-1, iNOS, MMP-9, IL-6, IL-1β, TNF-α mRNA were measured by RT-PCR. GAPDH levels were measured as a loading control. Data are representative of four such experiments, which gave similar results (**p<0.01 versus Control).
