Skip to main content
. 2013 Sep 3;54(9):5995–6002. doi: 10.1167/iovs.13-12117

Figure 5.

Figure 5

The PERK and ATF6 pathways are downregulated in ATF4+/− OIR retina. Retinal protein extracts were analyzed 24 hours after transferring mice to room air (P13). (A) Protein levels were analyzed in C57BL/6 OIR (n = 4) and ATF4+/− OIR (n = 4) retinas using a ratio of arbitrary units (a.u) in experimental groups to control groups. The Student's t-test was used to calculate differences in protein expression. While Bip and Chop protein expression was not modified in ATF4+/− OIR retinas, the levels of pIF2α (the hallmark of the PERK pathway), pATF6 (50 kD), and pATF6 (90 kD) (the ATF6 pathway) were significantly decreased in ATF4+/− OIR retinas by 20% (P = 0.02), 27% (P = 0.001), and 29% (P = 0.002), respectively. The VEGFa protein levels were reduced in ATF4+/− OIR retinas by 20% compared with controls (P = 0.005) (*P < 0.05, **P < 0.01). (B) Images of blots stained for pATF6, peIF2, VEGF, and β-actin.