Figure 3.

The gp100 APL S4S6 is able to induce T cell cytotoxicity. TCR-engineered T cells were tested in a ⁵¹Cr-release assay. T2 target cells were either non-loaded or loaded with titrated amounts of gp100 wt peptide (wt) or APLs A3 and S4S6 (range: 10⁻⁵–10⁻¹² M peptide). T cell cytotoxicity was assayed after 6 h with an effector to target cell ratio of 30:1, after which specific lysis was calculated. Results of one (out of two) representative experiment are shown based on the mean value of triplicate data points.