Figure 4.
The gp100 APL S4S6 induces a changed cytokine production profile. TCR-engineered T cells were tested for the production of IFNγ, TNFα, IL-2, IL-4, IL-5, and IL-10 using a Cytokine Bead Array (CBA). T2 target cells were either non-loaded or loaded with titrated amounts of gp100 wt peptide (wt) or APLs A3 and S4S6 (range: 10−5–10−12 M peptide). Following an 18 h co-cultivation at an effector to target cell ratio of 3:1, supernatants were collected and measured for cytokine content. Results of one (out of three) representative experiment are shown based on the mean value of triplicate data points.