Figure 5.
The gp100 APL S4S6 is not able to induce activation of NFAT. TCR-engineered Jurkat T cells were transfected with NFAT reporter and β-galactosidase constructs and subsequently co-cultured with T2 target cells that were either non-loaded or loaded with titrated amounts of gp100 wt peptide (wt) or APLs A3 and S4S6 (range: 10−5–10−12 M peptide). Following a 6 h co-cultivation at an effector to target cell ratio of 2:1, lysates were collected and measured for luciferase activities [depicted as light units (LU) corrected for medium only]. Results of one (out of two) representative experiment are shown based on the mean value of triplicate data points.