Figure 7.

The gp100 APL S4S6 renders the gp100/HLA-A2-specific TCR CD8 dependent. TCR-engineered T cells were tested in a ⁵¹Cr-release assay as described in legend to Figure 3 either without or with blocking anti-CD8 mAb (left and right panels, respectively). T2 cells were either non-loaded or loaded with titrated amounts of gp100 wt peptide (wt) or APLs A3 and S4S6 (range: 10⁻⁶–10⁻⁹ M peptide). Results of one (out of two) representative experiment are shown based on the mean value of triplicate data points.