Figure 3.

Interleukin 1 (IL-1) adjuvant increases the recruitment of antifungal T-helper 17 cells into the lung upon recall. A and B, The number (A) and percentage (B) of cytokine-producing 1807 transgenic and endogenous CD4⁺ T cells (A) that migrated to the lung at day 4 after infection are shown. Mice received 10⁶ adoptively transferred naive 1807 cells intravenously and were vaccinated with 10⁶ heat-killed yeast or 10⁵ live yeast (as a positive control) with or without recombinant IL-1β. Two weeks after the boost, mice were challenged with virulent wild-type yeast, and the number of cytokine-producing T cells was enumerated by fluorescence-activated cell sorter analysis. Data are mean ± standard error of the mean (n = 4–6 mice/group) and are representative of 2 experiments. *P < .05 vs all other groups. Abbreviations: IFN-γ, interferon γ; IL-13, interleukin 13; IL-17, interleukin 17.