Table 2.
[Na+], mM | ka, M−1 s−1 | kd, s−1 | KD, nM | ||
---|---|---|---|---|---|
Kinetic fit | Steady-state | Filter bindinga | |||
100 | 0.9b | 0.543 ± 0.153 | |||
150 | 3.3b | 2.7 ± 0.4 | |||
250 | (3.2 ± 0.1) × 104 | 0.00059 ± 0.00002 | 18 ± 1 | 18 ± 2 | 40 ± 5.5 |
300 | (1.0 ± 0.1) × 105 | 0.0026 ± 0.0001 | 26 ± 4 | 28 ± 3 | |
350 | (7.7 ± 0.1) × 105 | 0.027 ± 0.001 | 34 ± 1 | 45 ± 4 | |
400 | (2.4 ± 0.1) × 106 | 0.18 ± 0.01 | 73 ± 1 | 63 ± 2 | |
450 | 94.4 ± 21.2 | ||||
500 | 92 ± 7 | ||||
600 | 329 ± 11 |