. Author manuscript; available in PMC: 2014 May 27.

Published in final edited form as: J Mol Biol. 2013 Feb 14;425(10):1655–1669. doi: 10.1016/j.jmb.2013.02.010

Table 2.

SPR analysis of PU.1 ETS-DNA binding to the ?B site (GGAA-1) in the presence of salmon sperm DNA as nonspecific competitor.

[Na⁺], mM	k_a, M⁻¹ s⁻¹	k_d, s⁻¹	K_D, nM
[Na⁺], mM	k_a, M⁻¹ s⁻¹	k_d, s⁻¹	Kinetic fit	Steady-state	Filter binding^a
100				0.9^b	0.543 ± 0.153
150				3.3^b	2.7 ± 0.4
250	(3.2 ± 0.1) × 10⁴	0.00059 ± 0.00002	18 ± 1	18 ± 2	40 ± 5.5
300	(1.0 ± 0.1) × 10⁵	0.0026 ± 0.0001	26 ± 4	28 ± 3
350	(7.7 ± 0.1) × 10⁵	0.027 ± 0.001	34 ± 1	45 ± 4
400	(2.4 ± 0.1) × 10⁶	0.18 ± 0.01	73 ± 1	63 ± 2
450					94.4 ± 21.2
500				92 ± 7
600				329 ± 11

From Poon and Macgregor.¹⁸

Values extrapolated from the plot in Figure 4.

Errors listed in this table are the standard errors for the fit to the 1:1 model.